Dr. Felipe Opazo

Publications of Felipe Opazo

 

Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy.

Gomes de Castro MA, Höbartner C,Opazo F.
PLoS One. 2017 Feb 24;12(2):e0173050. doi: 10.1371/journal.pone.0173050. eCollection 2017.
View at PubMed


ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones.

Kawabe H, Mitkovski M, Kaeser PS, Hirrlinger J, Opazo F, Nestvogel D, Kalla S, Fejtova A, Verrier SE, Bungers SR, Cooper BH, Varoqueaux F, Wang Y, Nehring RB, Gundelfinger ED, Rosenmund C, Rizzoli SO, Südhof TC, Rhee JS, Brose N.
J Cell Biol. 2017 Apr 3;216(4):1143-1161. doi: 10.1083/jcb.201606086. Epub 2017 Mar 6.
View at PubMed


Correction: ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones.

Kawabe H, Mitkovski M, Kaeser PS, Hirrlinger J, Opazo F, Nestvogel D, Kalla S, Fejtova A, Verrier SE, Bungers SR, Cooper BH, Varoqueaux F, Wang Y, Nehring RB, Gundelfinger ED, Rosenmund C, Rizzoli SO, Südhof TC, Rhee JS, Brose N.
J Cell Biol. 2017 Apr 3;216(4):1205. doi: 10.1083/jcb.20160608603092017c. Epub 2017 Mar 13. No abstract available.
PMID: 28289090

View at PubMed


 

Aptamer Stainings for Super-resolution Microscopy.

de Castro MA, Rammner B, Opazo F.
Methods Mol Biol. 2016;1380:197-210. doi: 10.1007/978-1-4939-3197-2_17.
View at PubMed


Tools and limitations to study the molecular composition of synapses by fluorescence microscopy.

Maidorn M, Rizzoli SO, Opazo F
Biochem J. 2016 Oct 15;473(20):3385-3399. Review.
View at PubMed


 

Super-resolution imaging for cell biologists: concepts, applications, current challenges and developments.

Fornasiero EF, Opazo F.
Bioessays. 2015 Apr;37(4):436-51. doi: 10.1002/bies.201400170. Epub 2015 Jan 12.
View at PubMed


Secondary-ion mass spectrometry of genetically encoded targets.

Vreja IC, Kabatas S, Saka SK, Kröhnert K, Höschen C, Opazo F, Diederichsen U, Rizzoli SO.
Angew Chem Int Ed Engl. 2015 May 4;54(19):5784-8. doi: 10.1002/anie.201411692. Epub 2015 Mar 17.
View at PubMed


A contamination-insensitive probe for imaging specific biomolecules by secondary ion mass spectrometry.

Kabatas S, Vreja IC, Saka SK, Höschen C, Kröhnert K, Opazo F, Rizzoli SO, Diederichsen U.
Chem Commun (Camb). 2015 Aug 6;51(67):13221-4. doi: 10.1039/c5cc03895b.
View at PubMed


Resolving bundled microtubules using anti-tubulin nanobodies.

Mikhaylova M, Cloin BM, Finan K, van den Berg R, Teeuw J, Kijanka MM, Sokolowski M, Katrukha EA, Maidorn M, Opazo F, Moutel S, Vantard M, Perez F, van Bergen en Henegouwen PM, Hoogenraad CC, Ewers H, Kapitein LC.
Nat Commun. 2015 Aug 11;6:7933. doi: 10.1038/ncomms8933.
View at PubMed



Mapping molecules in scanning far-field fluorescence nanoscopy.

Ta H, Keller J, Haltmeier M, Saka SK, Schmied J, Opazo F, Tinnefeld P, Munk A, Hell SW.
Nat Commun. 2015 Aug 13;6:7977. doi: 10.1038/ncomms8977.
View at PubMed



Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer.

Opazo F, Eiden L, Hansen L, Rohrbach F, Wengel J, Kjems J, Mayer G.
Mol Ther Nucleic Acids. 2015 Sep 1;4:e251. doi: 10.1038/mtna.2015.25.
View at PubMed

 

Fluorescent in situ hybridization of synaptic proteins imaged with super-resolution STED microscopy.

Zhang WI, Röhse H, Rizzoli SO, Opazo F.
Microsc Res Tech. 2014 Apr 10. doi: 10.1002/jemt.22367.
View at PubMed


Cellular and Molecular Applications of Super-resolution Microscopy.

Opazo F, Rizzoli SO.
Fluorescence Microscopy: Super-Resolution and Other Novel Techniques. Academic Press 133-152. ISBN: 978-0-12-409513-7
Link to elsevier


Probing Biological Samples in High-Resolution Microscopy: Making Sense of Spots.

Opazo F
Super-Resolution Microscopy Techniques in the Neurosciences.Neuromethods, Vol 86:369-386. ISBN: 978-1-62703-982-6
Link to SpringerLink

 

Aptamers as potential tools for super-resolution microscopy.

Opazo F, Levy M, Byrom M, Schäfer C, Geisler C, Groemer TW, Ellington AD, Rizzoli SO.
Nat Methods. 2012 Oct;9(10):938-9. doi: 10.1038/nmeth.2179. No abstract available.
View at PubMed


An RNA alternative to human transferrin: a new tool for targeting human cells.

Wilner SE, Wengerter B, Maier K, de Lourdes Borba Magalhães M, Del Amo DS, Pai S, Opazo F, Rizzoli SO, Yan A, Levy M.
Mol Ther Nucleic Acids. 2012 May 15;1:e21. doi: 10.1038/mtna.2012.14.
View at PubMed

 

Endosomal sorting of readily releasable synaptic vesicles.

Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO.
Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):19055-60. doi: 10.1073/pnas.1007037107. Epub 2010 Oct 18.
View at PubMed


The fate of synaptic vesicle components upon fusion.

Opazo F, Rizzoli SO.
Commun Integr Biol. 2010 Sep;3(5):427-9. doi: 10.4161/cib.3.5.12132.
View at PubMed


PKC links Gq-coupled receptors to DAT-mediated dopamine release.

Opazo F, Schulz JB, Falkenburger BH.
J Neurochem. 2010 Jul;114(2):587-96. doi: 10.1111/j.1471-4159.2010.06788.x. Epub 2010 Apr 29.
View at PubMed


Aged Tgfbeta2/Gdnf double-heterozygous mice show no morphological and functional alterations in the nigrostriatal system.

Heermann S, Opazo F, Falkenburger B, Krieglstein K, Spittau B.
J Neural Transm. 2010 Jun;117(6):719-27. doi: 10.1007/s00702-010-0406-7. Epub 2010 May 11.
View at PubMed


Limited intermixing of synaptic vesicle components upon vesicle recycling.

Opazo F, Punge A, Bückers J, Hoopmann P, Kastrup L, Hell SW, Rizzoli SO.
Traffic. 2010 Jun;11(6):800-12. doi: 10.1111/j.1600-0854.2010.01058.x. Epub 2010 Mar 9.
View at PubMed


Studying synaptic vesicle pools using photoconversion of styryl dyes.

Opazo F, Rizzoli SO.
J Vis Exp. 2010 Feb 15;(36). doi:pii: 1790. 10.3791/1790.
View at PubMed

 

Pre-fibrillar alpha-synuclein variants with impaired beta-structure increase neurotoxicity in Parkinson’s disease models.

Karpinar DP, Balija MB, Kügler S, Opazo F, Rezaei-Ghaleh N, Wender N, Kim HY, Taschenberger G, Falkenburger BH, Heise H, Kumar A, Riedel D, Fichtner L, Voigt A, Braus GH, Giller K, Becker S, Herzig A, Baldus M, Jäckle H, Eimer S, Schulz JB, Griesinger C, Zweckstetter M.
EMBO J. 2009 Oct 21;28(20):3256-68. doi: 10.1038/emboj.2009.257. Epub 2009 Sep 10.
View at PubMed


Rapid microtubule bundling and stabilization by the Streptococcus pneumoniae neurotoxin pneumolysin in a cholesterol-dependent, non-lytic and Src-kinase dependent manner inhibits intracellular trafficking.

Iliev AI, Djannatian JR, Opazo F, Gerber J, Nau R, Mitchell TJ, Wouters FS.
Mol Microbiol. 2009 Jan;71(2):461-77. doi: 10.1111/j.1365-2958.2008.06538.x. Epub 2008 Nov 19.
View at PubMed

 

Accumulation and clearance of alpha-synuclein aggregates demonstrated by time-lapse imaging.

Opazo F, Krenz A, Heermann S, Schulz JB, Falkenburger BH.
J Neurochem. 2008 Jul;106(2):529-40. doi: 10.1111/j.1471-4159.2008.05407.x. Epub 2008 Apr 10.
View at PubMed


Membrane-permeable Bcl-xL prevents MPTP-induced dopaminergic neuronal loss in the substantia nigra.

Dietz GP1, Stockhausen KV, Dietz B, Falkenburger BH, Valbuena P, Opazo F, Lingor P, Meuer K, Weishaupt JH, Schulz JB, Bähr M.
J Neurochem. 2008 Feb;104(3):757-65. Epub 2007 Nov 6.
View at PubMed

 

Effects of natural flavones and flavonols on the kinase activity of Cdk5.

Zapata-Torres G, Opazo F, Salgado C, Muñoz JP, Krautwurst H, Mascayano C, Sepúlveda-Boza S, Maccioni RB, Cassels BK.
J Nat Prod. 2004 Mar;67(3):416-20.
View at PubMed

Copyright 2013 - 2017 | rizzoli-lab.de